The musical opened on Broadway on October 18, 1966 at the Shubert Theatre and ran for 463 performances, closing on November 25, 1967. It was produced by Stuart Ostrow, directed by Mike Nichols and starred Barbara Harris, Alan Alda, and Larry Blyden. Harris won the Tony Award for Best Actress in a Musical. The Apple Tree also received several other major Tony nominations: Bock and Harnick for Best Composer and Lyricist, Nichols for Best Direction of a Musical, Lee Theodore for Best Choreography, and the show itself for Best Musical.
The Encores! staged concert production ran from May 12, 2005 through May 16, 2005, and starred Kristin Chenoweth, Malcolm Gets and Michael Cerveris.
The Roundabout Theatre Company mounted a revival that ran from December 14, 2006 until March 11, 2007 with Kristin Chenoweth in Harris' roles, Brian D'Arcy James in Alda's roles and Marc Kudisch in Blyden's. The consensus of reviews was that the playlets themselves are all creaky with age, the music interesting but not inspired, and Chenoweth's performance a marvel and the evening's only important attraction. It ran for 18 previews and 99 regular shows for a total of 117 performances.